Brief Analysis of Methods for Cloud Computing Key Management

In this paper basic of cloud and possible methods for its key management is discussed. Now a days cloud computing is good arena in the field of research.  In cloud computing cloud customer and cloud provider needs to secure data against loss and theft. Encryption with key management is a technique for securing the personal and enterprise data. It is mainly used to protect data. In this paper how key management can be performed to protect cloud data is discussed. So that risks of data loss and theft can be reduced. Keywords: Cloud computing, Cloud architecture, Encryption, Key managemen

Review on Resource Efficient Relay Selection Scheme for Cognitive Radio Network

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Genome-wide transcriptome analysis and physiological variation modulates gene regulatory networks acclimating salinity tolerance in chickpea

Salinity is a major abiotic stress that is a global threat to crop production, including chickpea. This study focused on understanding the complex molecular mechanisms underlying salinity tolerance using comparative transcriptome analysis of tolerant (ICCV 10, JG 11) and sensitive (DCP 92-3, Pusa 256) chickpea genotypes in control and salt-stressed environments. A total of 530 million reads were generated from root samples of four genotypes using Illumina HiSeq-2500. A total of 21,698 differentially expressed genes (DEGs) were identified, of which 11,456 and 10,242 were up- and down-regulated, respectively, in comparative analysis. These DEGs were associated with crucial metabolic pathways, including hormone signaling, photosynthesis, lipid and carbohydrate metabolism, and cell wall biogenesis. Gene ontology (GO) examination revealed an enrichment of transcripts involved in salinity response. A total of 4257 differentially expressed GO terms were categorized into 64 functional groups; of which, GO terms like, integral component of membrane, organelle, and cellular anatomical entity were highly represented in tolerant genotypes under salt stress. Significant up-regulation of transcripts encoding potassium transporter family HAK/KUP proteins, MIP/aquaporin protein family, NADH dehydrogenase, pectinesterase, and PP2C family proteins occurred under salt stress. The tolerant lines (ICCV 10 and JG 11) engaged highly efficient machinery in response to elevated salt stress, especially for signal transduction, transport and influx of K+ ions, and osmotic homeostasis. The overall study highlights the role of potential candidate genes and their regulatory networks which can be utilized in breeding salt tolerant chickpea cultivars

Development and Validation of a Green Analytical Method for the Determination of Aspirin and Domperidone Bulk or Formulation Using UV and HPLC

The aim of present study was to investigate the development and validation of a green analytical method for the determination of aspirin and domperidone. Method Development and Validation for Estimation of Domperidone and Aspirin in bulk or formulation by using RP-HPLC. The RP-HPLC method was developed for estimation of Aspirin and Domperidone in synthetic mixture by isocratically using 10 mM KH2PO4: Acetonitrile (20:80) as mobile phase, Prontosil C-18 column (4.6 x 250 mm, 5μparticle size) column as stationary phase and chromatogram was recorded at 231 nm. Then developed method was validated by using various parameters such as, linearity, Range accuracy, precision repeatability, intermediate precision, robustness, limit of detection, limit of quantification. The proposed methods were found to be linear with correlation coefficient close to one. Precision was determined by repeatability, Intermediate precision and reproducibility of the drugs. The robustness of developed method was checked by changing in the deliberate variation in solvent. The result obtained shows the developed methods to be Cost effective, Rapid (Short retention time), Simple, Accurate (the value of SD and % RSD less than 2), Precise and can be successfully employed in the routine analysis of these drugs in bulk drug as well as in tablet dosage form. The Simplicity, Rapidly and Reproducibility of the proposed method completely fulfill the objective of this research work. Keywords: Asprin; Domperidone; HPLC; Ultra Violet; Validatio

Etiology and Considerations of Developmental Enamel Defects in Children: A Narrative Review

Context: Dental enamel is the hardest and highly mineralized structure in human body. However, Developmental Enamel Defects (DEDs) may occur due to an interplay between multiple factors ranging from genetic inadequacy to environmental insults. Primary enamel defects provoke the local or systemic insults that the child might undergo pre-, peri- and post-natally. Several gene mutations and environmental factors, including systemic illnesses have already been identified that can permanently imprint enamel damage. The DED may appear as enamel hypoplasia or hypomineralization. Clinically, DED often presents problems of aesthetics and stained defects, tooth sensitivity, susceptibility to dental caries, erosion and tooth wear.  Evidence Acquisition: An electronic search was conducted on PubMed, Cochrane, ScienceDirect and Clinical Key databases with the focus on articles published since 2000. The following keywords were applied: “Developmental Enamel Defect (DED)”, “Enamel hypoplasia”, and “Primary teeth”. Results: Managing the enamel defects involves early diagnosis and aesthetic rehabilitation of defective enamel, while maintaining its form and function. This should involve close cooperation between the paediatricians and the paediatric dentists, so that preventive regimens can be institutionalised at the earliest. Conclusions: Despite our understanding of DED, further research is required to establish accurate clinical diagnosis and successful treatment of such enamel defects

Nagpur.

Abstract- Research problems are to enhance an Intrusion Detection System (IDS) of a clustered HWSN to prolong its lifetime operation in the presence of unreliable and malicious nodes. Also, to address the energy consumption and QoS gain in reliability, delay and security with the goal to maximize the lifetime of a clustered HWSN while satisfying application QoS requirements in the context of multipath routing. The proposed research is a highly scalable cluster-based hierarchical trust management protocol for wireless sensor networks (WSNs) to effectively deal with selfish or malicious nodes. The proposed work consider multidimensional trust attributes derived from communication and social networks to evaluate the overall trust of a sensor node. System describes a heterogeneous WSN comprising a large number of sensor nodes with vastly different social and quality of service (QoS) behaviors with the objective t

Unconventional complete dentures: Innovative approach in prosthodontics

Abstract: Routine complications faced by the dentist include atrophic ridge, microstomia, flabby tissue, xerostomia , bony exostosis, labially inclined premaxilla, esthetic demand, bruxism, systemic disorders, patient's demand for duplicating dentures,etc. Management of these difficulties can be done by proper incorporating of suitable materials and advanced techniques. This article describes the unconventional approaches to various modalities so as to provide ultimate satisfaction for the patient

Global Epigenetic Changes Induced by SWI2/SNF2 Inhibitors Characterize Neomycin-Resistant Mammalian Cells

<div><h3>Background</h3><p>Previously, we showed that aminoglycoside phosphotransferases catalyze the formation of a specific inhibitor of the SWI2/SNF2 proteins. Aminoglycoside phosphotransferases, for example neomycin-resistant genes, are used extensively as selection markers in mammalian transfections as well as in transgenic studies. However, introduction of the neomycin-resistant gene is fraught with variability in gene expression. We hypothesized that the introduction of neomycin-resistant genes into mammalian cells results in inactivation of SWI2/SNF2 proteins thereby leading to global epigenetic changes.</p> <h3>Methodology</h3><p>Using fluorescence spectroscopy we have shown that the inhibitor, known as <u>A</u>ctive <u>D</u>NA-<u>d</u>ependent <u>A</u>TPase <u>A</u><u>D</u>omain inhibitor (ADAADi), binds to the SWI2/SNF2 proteins in the absence as well as presence of ATP and DNA. This binding occurs via a specific region known as Motif Ia leading to a conformational change in the SWI2/SNF2 proteins that precludes ATP hydrolysis. ADAADi is produced from a plethora of aminoglycosides including G418 and Streptomycin, two commonly used antibiotics in mammalian cell cultures. Mammalian cells are sensitive to ADAADi; however, cells stably transfected with neomycin-resistant genes are refractory to ADAADi. In resistant cells, endogenous SWI2/SNF2 proteins are inactivated which results in altered histone modifications. Microarray data shows that the changes in the epigenome are reflected in altered gene expression. The microarray data was validated using real-time PCR. Finally, we show that the epigenetic changes are quantized.</p> <h3>Significance</h3><p>The use of neomycin-resistant genes revolutionized mammalian transfections even though questions linger about efficacy. In this study, we have demonstrated that selection of neomycin-resistant cells results in survival of only those cells that have undergone epigenetic changes, and therefore, data obtained using these resistant genes as selection markers need to be cautiously evaluated.</p> </div

Expression of endogenous SG2NA is influenced by ADAADi production.

<p>The transcript as well as protein expression was monitored in the untransfected cells as well as in cells stably transfected with pcDNA 3.1 myc/his (−) vector at passage 13. (A). Endogenous SG2NA transcript was analyzed by quantitative RT-PCR in untransfected and transfected Neuro 2Acells. (B). Endogenous SG2NA protein analyzed by western blot using antibody against SG2NA. (C). <i>sg2na</i> promoter occupancy by RNAPII, Brg1, H3K9Ac, and H3K9Me2 was analysed in untransfected and transfected Neuro 2A cells using ChIP. Fold enrichment was calculated with respect to the mock ChIP done using IgG antibodies. (D). SG2NA transcript level in transfected cells at passage 4. (E). <b>Expression of exogenous SG2NA expressed using pcDNA 3.1 myc/his (−) vector </b><b>is also influenced by ADAADi production.</b> Overexpression of three variants of SG2NA in Neuro2A cells were monitored using anti-myc antibody. Transfected cells (passage 4) were grown as indicated for 12 hours before analysis. Two clones of 87 kDa (87.1 and 87.2), one clone each of 78 kDa (78.1) and of 52 kDa (52.2) were analyzed for protein expression. The cells transfected with vector alone were used as control. Protein expression was observed only when cells were grown in the absence of both antibiotics. (F). Western blot analysis of expression of 87- and 78-kDa proteins in clones 87.1 and 78.1 in stably transfected cells grown in the presence of antibiotics using anti-myc antibody. Lane 1: vector alone transfected cells; Lane 2: 78.1 clone; Lane 3: 87.1 clone. N.S., indicating non-specific band, was used as loading control. (G). The expression of 78- and 87- kDa protein in 78.1, 78.2, 87.1 and 87.2 clones was monitored in stably transfected cells grown in the absence of antibiotics. Lane 1: vector transfected cells; Lane 2: 78.1 clone; Lane 3: 78.2 clone; M: marker; Lane 4: 87.1 clone; Lane 5: 87.2 clone. N.S., indicating non-specific band, was used as loading control. (H). Semi-quantitative RT-PCR analysis done using insert-specific forward primer and vector-specific reverse primer confirms that 87 kDa transcript expression is observed only when the cells are grown the absence of antibiotics. (I). The expression of 87 kDa, 78 kDa, and 52 kDa was not observed in stably transfected cells even after removal of antibiotics when the cells were freeze-thawed at passage 9. (J). Semi-quantitative RT-PCR analysis of APH transcript. Lane1: Untransfected Neuro2A cells; Lane 2: Stably transfected Neuro2A cells; Lane 3: control reaction using purified pcDNA 3.1 myc/his (−) vector.</p